Hypomethylation of LINE-1 repeat elements and global loss of DNA hydroxymethylation in vapers and smokers.
Andrew W CaliriAmanda CaceresStella TommasiAhmad BesaratiniaPublished in: Epigenetics (2020)
5-mC: 5-methylcytosine; 5-hmC: 5-hydroxymethylcytosine; 8-OHdG: 8-hydroxy-2'-deoxyguanosine; ACTIN: actin beta; ANOVA: Analysis of Variance; BER: base excision repair; BMI: body mass index; CO: carbon monoxide; COHb: carboxyhaemoglobin; COBRA: combined bisulphite restriction analysis; COPD: chronic obstructive pulmonary disease; DNMT1: DNA methyltransferase 1; DNMT3A: DNA methyltransferase 3A; DNMT3B: DNA methyltransferase 3B; e-cigs: electronic cigarettes; ELISA: enzyme-linked immunosorbent assay; ENDS: electronic nicotine delivery systems; FDA: Food and Drug Administration; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; HPLC: high-performance liquid chromatography; LINE-1: Long Interspersed Nucleotide Element 1; PBS: phosphate-buffered saline; RFU: relative fluorescence units; RT-qPCR: quantitative reverse-transcription polymerase chain reaction; ROS: reactive oxygen species; SAM, S-adenosylmethionine; SE: standard error; TET1: ten-eleven translocation 1; TET2: ten-eleven translocation 2; TET3: ten-eleven translocation 3.
Keyphrases
- high performance liquid chromatography
- chronic obstructive pulmonary disease
- body mass index
- single molecule
- circulating tumor
- reactive oxygen species
- dna methylation
- smoking cessation
- cell free
- simultaneous determination
- tandem mass spectrometry
- solid phase extraction
- drug administration
- mass spectrometry
- lung function
- ms ms
- high resolution
- weight gain
- nucleic acid
- transcription factor
- replacement therapy
- circulating tumor cells
- dna damage
- physical activity
- risk assessment
- oxidative stress
- weight loss