An innovative chemical strategy for PCR-free genetic detection of pathogens by an integrated electrochemical biosensor.
Salvatore PetraliaEmanuele Luigi SciutoMaria Letizia Di PietroM ZimboneM G GrimaldiSebania LibertinoPublished in: The Analyst (2017)
An innovative chemical strategy integrated in a miniaturized electrochemical device was developed for sensitive detection of a pathogen genome (HBV virus) without any amplification step. The results show a limit of detection comparable to the standard qRT-PCR method (20 copies per reaction), paving the way to future development of genetic PoC devices addressing automatized and low-cost molecular diagnostics.
Keyphrases
- label free
- sensitive detection
- loop mediated isothermal amplification
- low cost
- real time pcr
- gold nanoparticles
- genome wide
- quantum dots
- hepatitis b virus
- copy number
- ionic liquid
- molecularly imprinted
- gene expression
- dna methylation
- gram negative
- single molecule
- electron transfer
- mass spectrometry
- tandem mass spectrometry