A ratiometric fluorescent probe for detection of exogenous mitochondrial SO 2 based on a FRET mechanism.

Zhiyang XuZhen ChenAikun LiuRuixue JiXiaoqun Cao Yanqing Ge

Published in: RSC advances (2019)

A novel imidazo[1,5- a ]pyridine-hemicyanine based ratiometric fluorescent probe for detection of mitochondrial SO 2 was designed and synthesized. The probe is based on a fluorescence resonance energy transfer (FRET) mechanism. It exhibits high selectivity and sensitivity towards SO 3 2- with a fast response time (3 min) and detection limit of 0.13 μM. Further, it showed low cytotoxicity and was successfully applied to image exogenous mitochondrial SO 2 in cells.

Keyphrases

fluorescent probe
living cells
energy transfer
oxidative stress
single molecule
quantum dots
loop mediated isothermal amplification
label free
real time pcr
induced apoptosis
machine learning
signaling pathway
cell cycle arrest
nitric oxide
hydrogen peroxide
structural basis