Determination of favipiravir in human plasma using homogeneous liquid-liquid microextraction followed by HPLC/UV.
Inas A AbdallahSherin F HammadAlaa BedairAhmed H ElshafeeyFotouh Rashed MansourPublished in: Bioanalysis (2022)
Background: Favipiravir is an antiviral drug that was recently approved for the management of COVID-19 infection. Aim: This work aimed to develop a new method, using sugaring-out induced homogeneous liquid-liquid microextraction followed by HPLC/UV for the determination of favipiravir in human plasma. Materials & methods: The optimum extraction conditions were attained using 500 μl of tetrahydrofuran as an extractant and 1400 mg of fructose as a phase-separating agent. Results: The developed method was validated according to the US FDA bioanalytical guidelines and was found linear in the range of 25-80,000 ng/ml with a correlation coefficient of 0.999. Conclusion: These results showed that the developed method was simple, easy, valid and adequately sensitive for determination of favipiravir in plasma for bioequivalence studies.
Keyphrases
- solid phase extraction
- high performance liquid chromatography
- molecularly imprinted
- simultaneous determination
- liquid chromatography tandem mass spectrometry
- tandem mass spectrometry
- liquid chromatography
- gas chromatography
- ms ms
- mass spectrometry
- diabetic rats
- magnetic resonance imaging
- clinical practice
- case control
- endothelial cells