Orbitrap mass spectrometry for monitoring the ganglioside pattern in human cerebellum development and aging.
Raluca IcaAlina PetrutCristian V A MunteanuMirela SarbuŽeljka VukelićLigia PetricaAlina Diana ZamfirPublished in: Journal of mass spectrometry : JMS (2020)
We have developed here a superior approach based on high-resolution (HR) mass spectrometry (MS) for monitoring the changes occurring with development and aging in the composition and structure of cerebellar gangliosidome. The experiments were focused on the comparative screening and structural analysis of gangliosides expressed in fetal and aged cerebellum by Orbitrap MS with nanoelectrospray ionization (nanoESI) in the negative ion mode. The employed ultrahigh-resolution MS platform allowed the discrimination, without the need of previous separation, of 159 ions corresponding to 120 distinct species in the native ganglioside mixtures from fetal and aged cerebellar biopsies, many more than detected before, when MS platforms of lower resolution were employed. A number of gangliosides, in particular polysialylated belonging to GT, GQ, GP, and GS classes, modified by O-fucosylation, O-acetylation, or CH3 COO- were discovered here, for the first time in human cerebellum. These components, found differently expressed in fetal and aged tissues, indicated that the ganglioside profile in cerebellum is development stage- and age-specific. Following the fragmentation analysis by high-energy collision-induced dissociation (HCD) tandem MS (MS/MS), we have also observed that the intimate structure of certain compounds has not changed during the development and aging of the brain, an aspect which could open new directions in the investigation of ganglioside biomarkers in cerebellar tissue.
Keyphrases
- mass spectrometry
- liquid chromatography
- high resolution
- ms ms
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- endothelial cells
- high resolution mass spectrometry
- multiple sclerosis
- tandem mass spectrometry
- ultra high performance liquid chromatography
- gene expression
- minimally invasive
- ionic liquid
- resting state
- liquid chromatography tandem mass spectrometry
- single molecule
- brain injury
- diabetic rats
- drug induced
- subarachnoid hemorrhage