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Reversible On-Off Photoswitching of DNA Replication Using a Dumbbell Oligodeoxynucleotide.

Yu WangHeming JiJian MaHang LuoYujian HeXinjing TangLi Wu
Published in: Molecules (Basel, Switzerland) (2022)
In most organisms, DNA extension is highly regulated; however, most studies have focused on controlling the initiation of replication, and few have been done to control the regulation of DNA extension. In this study, we adopted a new strategy for azODNs to regulate DNA extension, which is based on azobenzene oligonucleotide chimeras regulated by substrate binding affinity, and the conformation of the chimera can be regulated by a light source with a light wavelength of 365 nm. The results showed that the primer was extended with Taq DNA polymerase after visible light treatment, and DNA extension could be effectively hindered with UV light treatment. We also verify the reversibility of the photoregulation of primer extension through photoswitching of dumbbell asODNs by alternate irradiation with UV and visible light. Our method has the advantages of fast and simple, green response and reversible operations, providing a new strategy for regulating gene replication.
Keyphrases
  • circulating tumor
  • visible light
  • cell free
  • single molecule
  • nucleic acid
  • gene expression
  • genome wide
  • photodynamic therapy
  • combination therapy
  • copy number
  • gram negative