Structural Characterization and Biological Evaluation of 18-Nor-ent-labdane Diterpenoids from Grazielia gaudichaudeana.
Rodolfo B BalbinotJosiane A M de OliveiraDarlon I BernardiUlisses Z MeloÉrica B ZanquetaEliana H EndoFabianne M RibeiroHélito VolpatoMariana C FigueiredoDavi F BackErnani A BassoAna L T G RuizJoão E de CarvalhoMary Ann FoglioBenedito D Prado FilhoTania U NakamuraCelso V NakamuraMarta R B do CarmoMaria H SarragiottoDebora Cristina BaldoquiPublished in: Chemistry & biodiversity (2019)
The phytochemical investigation of Grazielia gaudichaudeana aerial parts yielded 15 compounds, including diterpenes, triterpenes, sterols and flavonoids. With exception to ent-kaurenoic acid diterpenes, the compounds isolated are being described for the first time in this species. Some unusual 1 H-NMR chemical shifts of 18-nor-ent-labdane (7-9) led us carry out a conformational analysis by theoretical calculations in order to support the experimental data. Moreover, due to the limitation of studies focused on pharmacological potential of Grazielia gaudichaudeana, the present study was carried out to investigate the antioxidant, antiproliferative, antiviral, antileishmanial and antimicrobial activities from the extract, fractions and isolated compounds obtained from this species. Ethyl acetate fraction showed significant activity in the antiproliferative assay, with GI50 range of 3.9 to 27.2 μg mL-1 . Dichloromethane fraction, rich in diterpenoids, inhibited all human tumor cell lines tested, and the nor-labdane 7 showed potent cytotoxic activity against glioma and ovary cancer cell lines.
Keyphrases
- anti inflammatory
- molecular dynamics simulations
- molecular dynamics
- oxidative stress
- endothelial cells
- papillary thyroid
- magnetic resonance
- staphylococcus aureus
- high throughput
- squamous cell
- induced pluripotent stem cells
- genetic diversity
- single molecule
- density functional theory
- squamous cell carcinoma
- climate change
- ionic liquid
- machine learning
- data analysis
- young adults
- lymph node metastasis