Spinning disk-remote focusing microscopy.
Michele GintoliSharika MohananPatrick S SalterElizabeth WilliamsJames D BeardGaspar JekelyAlexander David CorbettPublished in: Biomedical optics express (2020)
Fast confocal imaging was achieved by combining remote focusing with differential spinning disk optical sectioning to rapidly acquire images of live samples at cellular resolution. Axial and lateral full width half maxima less than 5 µm and 490 nm respectively are demonstrated over 130 µm axial range with a 256 × 128 µm field of view. A water-index calibration slide was used to achieve an alignment that minimises image volume distortion. Application to live biological samples was demonstrated by acquiring image volumes over a 24 µm axial range at 1 volume/s, allowing for the detection of calcium-based neuronal activity in Platynereis dumerilii larvae.
Keyphrases
- high resolution
- deep learning
- optical coherence tomography
- single molecule
- high speed
- label free
- convolutional neural network
- solid state
- photodynamic therapy
- minimally invasive
- machine learning
- high throughput
- loop mediated isothermal amplification
- mass spectrometry
- blood brain barrier
- zika virus
- raman spectroscopy
- fluorescence imaging
- brain injury
- subarachnoid hemorrhage
- sensitive detection