Real-time tracing the changes in the intracellular pH value during apoptosis by near-infrared ratiometric fluorescence imaging.
Liyun LinJingjin ZhaoLiang-Liang ZhangYong HuangFanggui YeShu-Lin ZhaoPublished in: Chemical communications (Cambridge, England) (2018)
We developed a near-infrared ratiometric fluorescent nanoprobe with single-excitation dual-emission. The proposed nanoprobe was applied for real-time monitoring the changes in the pH value during hydrogen peroxide-induced apoptosis by ratio imaging. The pH value of HeLa cells changed from 6.6 to 5.6 after H2O2 (100 μM) stimulation for 40 min.
Keyphrases
- induced apoptosis
- hydrogen peroxide
- endoplasmic reticulum stress
- living cells
- fluorescence imaging
- oxidative stress
- fluorescent probe
- cell cycle arrest
- signaling pathway
- quantum dots
- nitric oxide
- photodynamic therapy
- single molecule
- cell death
- high resolution
- energy transfer
- pi k akt
- sensitive detection
- cell proliferation