Characterization of reverse engineered anti-PF4 stereotypic antibodies derived from serum of VITT patients.
Jing Jing WangMarijn van der Neut KolfschotenLucy RuttenBridie Louise ArmourChee Wee TanTim ChatawayRinke BosAnnemart KoornneefPravien AbeywickremaRick KapurLeendert PorcelijnMidia Nouri KhalifaAva SadiPascale BouchierEleni KourkoutaAditya PerkasaTed Hj KwaksRoland C ZahnLaura SolforosiTom Paul GordonPublished in: Blood (2023)
This study presents a novel reverse-engineering approach to generate recombinant anti-PF4 antibodies from VITT-patient sera. These antibodies recapitulate functional effects of original serum anti-PF4 clonotypes, identify key PF4 binding residues and hold promise as valuable tools to investigate VITT etiology.