Isolation and complete genome sequencing of the virulent phage vB_EcoS_XY3 infecting multidrug-resistant Escherichia coli.
Pan FuQiang ZhaoLi ShiQin XiongHongxia XuSenmao CaiQianqian XuXiaodong SunMing SangPublished in: Archives of virology (2020)
A virulent phage, named vB_EcoS_XY3, was isolated from hospital wastewater in Xiangyang, China. Its morphological characteristics, growth parameters, adsorption rate, and pH and temperature stability were determined. Phage vB_EcoS_XY3 was found to be able to infect Escherichia coli laboratory strains and also some multidrug-resistant E. coli strains. Its complete genome consists of 51,345 base pairs of double-stranded DNA with an average GC content of 55.24% and 85 putative protein-coding genes. Forty-four genes were annotated with known functions. These results will not only provide further insights into E. coli phages but also have implications for the development of potential biocontrol agents.
Keyphrases
- escherichia coli
- multidrug resistant
- genome wide
- pseudomonas aeruginosa
- klebsiella pneumoniae
- acinetobacter baumannii
- drug resistant
- gram negative
- biofilm formation
- binding protein
- dna methylation
- healthcare
- genome wide identification
- bioinformatics analysis
- circulating tumor
- cell free
- wastewater treatment
- single molecule
- adverse drug
- genome wide analysis
- climate change
- protein protein
- transcription factor
- electronic health record
- gas chromatography