Mechanism Analysis of a Novel Angiotensin-I-Converting Enzyme Inhibitory Peptide from Isochrysis zhanjiangensis Microalgae for Suppressing Vascular Injury in Human Umbilical Vein Endothelial Cells.
Jiali ChenLi TanChengyong LiChunxia ZhouPengzhi HongShengli SunZhong-Ji QianPublished in: Journal of agricultural and food chemistry (2020)
Microalgae are primary producers with multiple nutrients in aquatic environments and mostly have applications in biological feed and fuel industry. There are few studies assessing the angiotensin-I-converting enzyme (ACE) inhibition potential of Isochrysis zhanjiangensis, other than its antioxidant potential. In this study, we evaluated a peptide from I. zhanjiangensis (PIZ, FEIHCC) and its vascular endothelial factors and mechanism in human umbilical vein endothelial cells (HUVEC). The results reveal that PIZ (IC50 = 61.38 μM) acts against ACE in a non-competitive binding mode. In addition, PIZ inhibits angiotensin II (Ang II)-induced vascular factor secretion and expression by blocking inflammation and apoptosis through nuclear factor κB (NF-κB), nuclear erythroid 2-related factor 2 (Nrf2), mitogen-activated protein kinases (MAPKs), and the serine/threonine kinase (Akt) signal pathways. This study reveals that PIZ has potential to be developed as a therapeutic agent for hypertension and provides a new method of high-value utilization of I. zhanjiangensis.
Keyphrases
- angiotensin ii
- angiotensin converting enzyme
- endothelial cells
- oxidative stress
- nuclear factor
- vascular smooth muscle cells
- high glucose
- signaling pathway
- toll like receptor
- blood pressure
- protein kinase
- diabetic rats
- cell proliferation
- human health
- immune response
- genome wide
- vascular endothelial growth factor
- pi k akt
- anti inflammatory