An origami paper-based analytical device for DNA damage analysis.
Wei Xuenull Dan ZhaoQiang ZhangYangyang ChangMeng LiuPublished in: Chemical communications (Cambridge, England) (2021)
Detection and characterization of DNA damage plays a critical role in genotoxicity testing, drug screening, and environmental health. We developed a fully integrated origami paper-based analytical device (oPAD) for measuring DNA damage. This simple device allows on-paper cell lysis, DNA extraction, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) reaction and signal readout with simple operation steps, enabling rapid (within 30 min) and high throughput assessment of multiple DNA damages induced by exogenous chemical agents.
Keyphrases
- dna damage
- high throughput
- dna repair
- oxidative stress
- circulating tumor
- single cell
- loop mediated isothermal amplification
- cell free
- single molecule
- public health
- healthcare
- liquid chromatography
- mental health
- cell therapy
- human health
- stem cells
- real time pcr
- circulating tumor cells
- label free
- climate change
- social media
- health promotion