RAA Enzyme Is a New Family of Class A Extended-Spectrum β-Lactamase from Riemerella anatipestifer Strain RCAD0122.

Hongyan LuoDekang ZhuMengru LiYunhan TangWenyu ZhangHaoju WangPei Li

Published in: Antimicrobial agents and chemotherapy (2022)

Whole-genome sequencing of Riemerella anatipestifer isolate RCAD0122 revealed a chromosomally located β-lactamase gene, bla RAA-1 , which encoded a novel class A extended-spectrum β-lactamase (ESBL), RAA-1. RAA-1 shared ≤65% amino acid sequence identity with other characterized β-lactamases. The kinetic assay of native purified RAA-1 revealed ESBL-like hydrolysis activity. Furthermore, bla RAA-1 could be transferred to a homologous strain by natural transformation. However, an epidemiological study showed that the bla RAA-1 gene is not prevalent currently.

Keyphrases

klebsiella pneumoniae
escherichia coli
multidrug resistant
amino acid
gram negative
copy number
genome wide
single cell
gene expression
dna repair
oxidative stress