The Role of Prep1 in the Regulation of Mesenchymal Stromal Cells.
Giorgia MaroniDaniele PanettaRaffaele LuongoIndira KrishnanFederica La RosaDaniela CampaniPiero SalvadoriPatricia IozzoFrancesco BlasiDmitry PenkovElena LevantiniMaria Cristina MagliPublished in: International journal of molecular sciences (2019)
Molecular mechanisms governing cell fate decision events in bone marrow mesenchymal stromal cells (MSC) are still poorly understood. Herein, we investigated the homeobox gene Prep1 as a candidate regulatory molecule, by adopting Prep1 hypomorphic mice as a model to investigate the effects of Prep1 downregulation, using in vitro and in vivo assays, including the innovative single cell RNA sequencing technology. Taken together, our findings indicate that low levels of Prep1 are associated to enhanced adipogenesis and a concomitant reduced osteogenesis in the bone marrow, suggesting Prep1 as a potential regulator of the adipo-osteogenic differentiation of mesenchymal stromal cells. Furthermore, our data suggest that in vivo decreased Prep1 gene dosage favors a pro-adipogenic phenotype and induces a "browning" effect in all fat tissues.
Keyphrases
- men who have sex with men
- bone marrow
- single cell
- mesenchymal stem cells
- high throughput
- cell fate
- high fat diet induced
- transcription factor
- cell proliferation
- genome wide
- rna seq
- type diabetes
- gene expression
- signaling pathway
- risk assessment
- dna methylation
- deep learning
- decision making
- human health
- single molecule