Optimized replication of arrayed bacterial mutant libraries increase access to biological resources.
Julia L E WillettAaron M T BarnesDebra N BrunsonAlexandre LecomteEthan B RobertsonGary M DunnyPublished in: bioRxiv : the preprint server for biology (2023)
Arrayed mutant collections drive robust genetic screens, yet few protocols exist for replication of these resources and subsequent quality control. Increasing distribution of arrayed biological collections will increase accessibility to and use of these resources. Developing standardized techniques for replication of these resources is essential for ensuring their quality and usefulness to the scientific community.