Myocardial infarction is a worldwide disease with high morbidity and mortality and a major cause of chronic heart failure, seriously affecting patients' quality of life. Natural medicine has been used to cure or prevent cardiovascular disease for decades. As a natural flavonoid, anthocyanidin has been used to treat many diseases due to its antioxidative, anti-inflammatory, and other properties. A mouse model (C57BL/6) weighing 30-40 g was utilized to induce myocardial infarction by ligating the left anterior descending coronary artery. Cyanidin (30 mg/kg) was administered orally to mice for four weeks. A variety of assessments were used to evaluate cardiac function. The gene expression was measured using RNAseq and Western blot. Histological changes in myocardial tissue were assessed using staining techniques, including Masson, Hematoxylin Eosin (HE), and transmission electron microscopy. Tunnel staining was implemented as a method to detect cellular apoptosis. For the quantification of B-type natriuretic peptide (BNP) and atrial natriuretic peptide (ANP) in the serum, an enzyme-linked immunosorbent assay (ELISA) was employed. Furthermore, autodock simulation was executed in order to assess the interaction between cyanidin and a subset of genes. Cyanidin treatment inhibited myocardial cell apoptosis, improved cardiac function, and reduced serum concentrations of BNP and atrial natriuretic peptide ANP, as well as mitigated histological cardiac tissue damage. Cyanidin also inhibited the activity of matrix metalloproteinases (MMP2/9) and Fibronectin 1 (Fn1). Cyanidin improves heart function and reduces myocardial damage in mice after MI. Furthermore, cyanidin can prevent cardiomyocyte apoptosis. These effects are most likely caused by suppression of MMP9/2 and control of the Akt signaling pathway, suggesting an appropriate therapeutic target.
Keyphrases
- left ventricular
- oxidative stress
- signaling pathway
- gene expression
- cardiovascular disease
- coronary artery
- mouse model
- endoplasmic reticulum stress
- cell cycle arrest
- ejection fraction
- heart failure
- high fat diet induced
- atrial fibrillation
- anti inflammatory
- cell death
- end stage renal disease
- left atrial
- pi k akt
- electron microscopy
- type diabetes
- dna methylation
- induced apoptosis
- chronic kidney disease
- angiotensin ii
- pulmonary artery
- coronary artery disease
- prognostic factors
- patient reported outcomes
- cell migration
- catheter ablation
- wild type
- patient reported
- high glucose
- genome wide
- insulin resistance
- transcription factor
- type iii