Genome-driven discovery of new serrawettin W2 analogues from Serratia fonticola DSM 4576.
Haolin QiuYang XiaoLing ShenTao HanQiang HeAiying LiPeng ZhangXiaofeng CaiPublished in: Organic & biomolecular chemistry (2023)
By expressing a multimodular NRPS gene sefA from Serratia fonticola DSM 4576 in E. coli , four new serrawettin W2 analogues, namely sefopeptides A-D (1-4), were isolated and structurally characterized and their biosynthesis was proposed. A bioactivity assay showed that sefopeptide C (3) exhibits moderate cytotoxic activity against acute promyelocytic leukemia NB4 cells.
Keyphrases
- induced apoptosis
- molecular docking
- high throughput
- genome wide
- liver failure
- cell cycle arrest
- escherichia coli
- small molecule
- acute myeloid leukemia
- bone marrow
- structure activity relationship
- respiratory failure
- copy number
- high intensity
- endoplasmic reticulum stress
- drug induced
- cell death
- gene expression
- genome wide identification
- oxidative stress
- dna methylation
- intensive care unit
- cell proliferation
- molecular dynamics simulations