Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216(T).
Elleke F BosmaJasper J KoehorstSacha A F T van HijumBernadet RenckensBastienne VriesendorpAntonius H P van de WeijerPeter J SchaapWillem M de VosJohn van der OostRichard van KranenburgPublished in: Standards in genomic sciences (2016)
Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of the B. smithii type strain DSM 4216(T), which consists of a 3,368,778 bp chromosome (GenBank accession number CP012024.1) and a 12,514 bp plasmid (GenBank accession number CP012025.1), together encoding 3880 genes. Genome annotation via RAST was complemented by a protein domain analysis. Some unique features of B. smithii central metabolism in comparison to related organisms included the lack of a standard acetate production pathway with no apparent pyruvate formate lyase, phosphotransacetylase, and acetate kinase genes, while acetate was the second fermentation product.
Keyphrases
- anaerobic digestion
- genome wide
- copy number
- escherichia coli
- sewage sludge
- microbial community
- bioinformatics analysis
- genome wide identification
- bacillus subtilis
- wastewater treatment
- amino acid
- crispr cas
- tyrosine kinase
- binding protein
- magnetic resonance imaging
- gene expression
- protein protein
- risk assessment
- diffusion weighted imaging
- small molecule
- transcription factor
- heavy metals
- drug induced