A yeast two-hybrid system for the screening and characterization of small-molecule inhibitors of protein-protein interactions identifies a novel putative Mdm2-binding site in p53.
Jin Huei WongMohammad AlfatahMei Fang SinHong May SimChandra S VermaDavid P LanePrakash ArumugamPublished in: BMC biology (2017)
The Y2H assay can be utilized for high-throughput screening of small-molecule inhibitors of PPIs and to identify domains that stabilize PPIs.