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Alkaline peptone water enrichment with a dipstick test to quickly detect and monitor cholera outbreaks.

Godfrey BwireChristopher Garimoi OrachDauda AbdallahAmanda Kay DebesAtek KagiritaMalathi RamDavid A Sack
Published in: BMC infectious diseases (2017)
Our findings showed that the modified dipstick test after enrichment with 1% APW had high level of accuracy in detection of V. cholerae and is quick, affordable alternative cholera outbreak monitoring tool in resource constrained settings. However, culture method should remain for cholera epidemic confirmation, for monitoring of antibiotic sensitivity and for production of pure isolates for molecular characterization. Further studies should be done to better understand the observed age and sex case distribution, in Kasese district.
Keyphrases
  • loop mediated isothermal amplification
  • sensitive detection
  • south africa
  • label free
  • real time pcr
  • infectious diseases