Dynamics of the transcriptome during chicken embryo development based on primordial germ cells.
Aleksandra DunislawskaAgata SzczerbaMaria SiwekMarek BednarczykPublished in: BMC research notes (2020)
Between 8 and 12 days of incubation, the highest number of genes was regulated. These data indicate that the most intense biological activity occurs between 8 and 12 days of embryo development. Heat map showed a significant decrease in gene expression on day 8, while it increased on day 12. The development of a precise method to isolate bird PGCs as well as the method to isolate RNA from single cells isolated from one embryo allows for early molecular analysis and detection of transcriptome changes during embryonic development.
Keyphrases
- gene expression
- induced apoptosis
- genome wide
- cell cycle arrest
- dna methylation
- single cell
- cell death
- transcription factor
- endoplasmic reticulum stress
- machine learning
- pregnancy outcomes
- electronic health record
- big data
- cell proliferation
- loop mediated isothermal amplification
- real time pcr
- bioinformatics analysis