In-cell chemical construction of a photoswitchable CENP-E using a photochromic covalent inhibitor.

Kazuya MatsuoShusuke YamaokaTomonori WakuAkio Kobori

Published in: Organic & biomolecular chemistry (2024)

An arylazopyrazole-based covalent inhibitor targeting the mitotic motor protein of centromere-associated protein E (CENP-E) was developed. Using this photoswitchable inhibitor, a photoswitchable CENP-E was chemically constructed in cells, which enabled to local control of mitotic cell division with light illumination.

Keyphrases

single cell
cell therapy
cell cycle
induced apoptosis
cell cycle arrest
wastewater treatment
oxidative stress
small molecule
signaling pathway
mesenchymal stem cells
cell proliferation
amino acid
drug delivery
pi k akt
endoplasmic reticulum stress