Sr-HA-graft-Poly(γ-benzyl-l-glutamate) Nanocomposite Microcarriers: Controllable Sr2+ Release for Accelerating Osteogenenisis and Bony Nonunion Repair.
Long GaoZhongyue HuangShifeng YanKunxi ZhangShenghua XuGuifei LiLei CuiJingbo YinPublished in: Biomacromolecules (2017)
The microcarrier system offers an attractive method for cellular amplification and phenotype enhancement in the field of bone tissue engineering. However, it remains a challenge to fabricate porous microcarriers with osteoinductive activity for speedy and high-quality osseointegration in regeneration of serious complication of bone fracture, like nonunion. Here, we present a facile method for the first time manufacture microcarriers with osteogenic effects and properties based on well controlled and long-term Sr2+ release. At first, strontium-substituted hydroxyapatite was prepared (Sr-HA) and a novel Sr-HA-graft-poly(γ-benzyl-l-glutamate) (Sr-HA-PBLG) nanocomposite was synthesized. Then, the microcarriers with highly interconnected macropores were fabricated by a double emulsion method, which allowed cells to adhere and proliferate and secrete extracellular matrix. Besides, the microcarriers with a relatively uniform diameter of 271.5 ± 45.0 μm are feasible for injection. The Sr-HA-PBLG microcarriers efficiently promoted osteogenic gene expression in vitro. With injection of the Sr-HA-PBLG microcarriers loading adipose derived stem cells (ADSCs) into the nonunion sites, bone regeneration was observed at 8 weeks after injection in a mice model.
Keyphrases
- bone regeneration
- tissue engineering
- gene expression
- extracellular matrix
- mesenchymal stem cells
- bone marrow
- stem cells
- ultrasound guided
- reduced graphene oxide
- induced apoptosis
- type diabetes
- quantum dots
- bone mineral density
- oxidative stress
- molecular docking
- high resolution
- cell proliferation
- cell death
- signaling pathway
- body composition
- skeletal muscle
- carbon nanotubes
- hip fracture
- solid phase extraction
- wild type
- pi k akt