A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors.
Isabel M L SaurSaskia BauerXunli LuPaul Schulze-LefertPublished in: Plant methods (2019)
Our data demonstrate that the here described approach can be effectively used to detect and quantify death of wheat and barley cells induced by overexpression of NLR and AVR effectors or AVR effector candidate genes from diverse fungal pathogens within 24 h.
Keyphrases
- cell death
- cell cycle arrest
- type iii
- induced apoptosis
- regulatory t cells
- dendritic cells
- cell proliferation
- high throughput
- electronic health record
- pi k akt
- cell wall
- gram negative
- transcription factor
- candida albicans
- endoplasmic reticulum stress
- signaling pathway
- antimicrobial resistance
- bioinformatics analysis
- artificial intelligence
- single cell
- data analysis