BaRTv1.0: an improved barley reference transcript dataset to determine accurate changes in the barley transcriptome using RNA-seq.

Paulo Rapazote-FloresMicha BayerLinda MilneClaus-Dieter MayerJohn FullerWenbin GuoPete E HedleyJenny MorrisClaire HalpinJason KamSarah M McKimMonika ZwirekM Cristina CasaoAbdellah BarakateMiriam SchreiberGordon StephenRunxuan ZhangJohn W S BrownRobbie WaughCraig G Simpson

Published in: BMC genomics (2019)

A high confidence barley reference transcript dataset consisting of 60,444 genes with 177,240 transcripts has been generated. Compared to current barley transcripts, BaRTv1.0 transcripts are generally longer, have less fragmentation and improved gene models that are well supported by splice junction reads. Precise transcript quantification using BaRTv1.0 allows routine analysis of gene expression and AS.

Keyphrases

rna seq
single cell
gene expression
genome wide
dna methylation
genome wide identification
copy number
clinical practice
transcription factor