Engineering of Escherichia coli for Krebs cycle-dependent production of malic acid.

Jean Marie FrançoisClément AuriolAudrey BaylacRomain IragueClémentine DressaireMarc Carnicer-HerasStéphanie HeuxJean Marie FrançoisThomas Walther

Published in: Microbial cell factories (2018)

Since more NAD(P)H and ATP cofactors are generated in the Krebs cycle-dependent malate production when compared to pathways which depend on the function of anaplerotic PEP carboxylase or PEP carboxykinase enzymes, the engineered strain developed in this study can serve as a platform to increase biosynthesis of malate-derived metabolites such as 2,4-dihydroxybutyric acid.

Keyphrases

escherichia coli
ms ms
high throughput
biofilm formation
cell wall
candida albicans