Sensitive and specific serological ELISA for the detection of SARS-CoV-2 infections.

Ji LuoAlexandra BrakelAndor KrizsanTobias LudwigMarina MötzingDaniela VolkeNicole LakowaThomas GrünewaldClaudia LehmannJohannes WolfStephan BorteSanja Milkovska-StamenovaJörg GabertFelix FingasMarkus ScholzRalf Hoffmann

Published in: Virology journal (2022)

We conclude that the N-protein-based ELISA developed here is well suited for the sensitive and specific serological detection of SARS-CoV-2 specific IgG antibodies in human serum for symptomatic infections. It may also prove useful to identify previous SARS-CoV-2 infections in vaccinated people, as all currently approved vaccines rely on the SARS-CoV-2 spike (S-) protein.

Keyphrases

sars cov
respiratory syndrome coronavirus
loop mediated isothermal amplification
label free
binding protein
amino acid
coronavirus disease
quantum dots