Stimulation of 3D osteogenesis by mesenchymal stem cells using a nanovibrational bioreactor.
Penelope M TsimbouriPeter G ChildsGabriel D PembertonJingli YangVineetha JayawarnaWich OrapiriyakulKarl BurgessCristina González-GarcíaGavin BlackburnDilip ThomasCatalina Vallejo-GiraldoManus J P BiggsAdam S G CurtisManuel Salmerón-SánchezStuart ReidMatthew J DalbyPublished in: Nature biomedical engineering (2017)
Bone grafts are one of the most commonly transplanted tissues. However, autologous grafts are in short supply, and can be associated with pain and donor-site morbidity. The creation of tissue-engineered bone grafts could help to fulfil clinical demand and provide a crucial resource for drug screening. Here, we show that vibrations of nanoscale amplitude provided by a newly developed bioreactor can differentiate a potential autologous cell source, mesenchymal stem cells (MSCs), into mineralized tissue in 3D. We demonstrate that nanoscale mechanotransduction can stimulate osteogenesis independently of other environmental factors, such as matrix rigidity. We show this by generating mineralized matrix from MSCs seeded in collagen gels with stiffness an order of magnitude below the stiffness of gels needed to induce bone formation in vitro. Our approach is scalable and can be compatible with 3D scaffolds.
Keyphrases
- mesenchymal stem cells
- bone regeneration
- cell therapy
- umbilical cord
- bone marrow
- wastewater treatment
- bone mineral density
- atomic force microscopy
- chronic pain
- tissue engineering
- platelet rich plasma
- gene expression
- neuropathic pain
- pain management
- stem cells
- human health
- bone loss
- soft tissue
- postmenopausal women
- spinal cord injury
- risk assessment
- resting state
- high resolution
- postoperative pain