Silk Protein-Mediated Biomineralization: From Bioinspired Strategies and Advanced Functions to Biomedical Applications.

Biomineralization refers to the process through which minerals nucleate in a structured manner to form specific crystal structures by the regulating of biomacromolecules. Biomineralization occurs in bones and teeth within the human body, where collagen acts as a template for the nucleation of hydroxyapatite (HA) crystals. Similar to collagen, silk proteins spun by silkworms can also serve as templates for the nucleation and growth of inorganic substances at interfaces. By enabling the binding of silk proteins to inorganic minerals, the process of biomineralization enhances the properties of silk-based materials and broadens their potential applications, rendering them highly promising for use in biomedical applications. In recent years, the development of biomineralized materials using silk proteins has garnered considerable attention in the biomedical field. This comprehensive review outlines the mechanism of biomineral formation mediated by silk proteins, as well as various biomineralization methods used to prepare silk-based biomineralized materials (SBBMs). Additionally, we discuss the physicochemical properties and biological functions of SBBMs, and their potential applications in various fields such as bioimaging, cancer therapy, antibacterial treatments, tissue engineering, and drug delivery. In conclusion, this review highlights the significant role that SBBMs can play in the biomedical field.