Single-Layered Graphene/Au-Nanoparticles-Based Love Wave Biosensor for Highly Sensitive and Specific Detection of Staphylococcus aureus Gene Sequences.
Junwang JiYiquan PangDongxiao LiXiaoli WangYi XuXiaojing MuPublished in: ACS applied materials & interfaces (2020)
In this work, a single-layered graphene (SLG)/Au-nanoparticles (NPs)-based Love wave biosensor was prepared for detection of Staphylococcus aureus (S. aureus) gene sequences. The annealing process was proposed to obtain a larger-area interface with ssDNA. The sensitivity was verified by detecting S. aureus gene sequences with a linear detection ranging from 0 to 10 nmol/L, where the limit of detection (LOD) was only 12.4 pg/mL. The stable state of the biosensors based on SLG/Au NPs in the liquid phase could be kept for more than 0.5 h (±0.1°), and the specificity was verified by detecting noncomplementary ssDNA and one- and three-base mismatched ssDNA. The results of our study suggest that a Love wave biosensor based on SLG/Au NPs hold great potential in clinical testing and diagnosis.
Keyphrases
- label free
- sensitive detection
- loop mediated isothermal amplification
- staphylococcus aureus
- reduced graphene oxide
- gold nanoparticles
- real time pcr
- quantum dots
- copy number
- genome wide
- gene expression
- biofilm formation
- mass spectrometry
- escherichia coli
- room temperature
- risk assessment
- pseudomonas aeruginosa
- walled carbon nanotubes
- highly efficient
- high resolution
- climate change
- carbon nanotubes
- ionic liquid
- transcription factor
- simultaneous determination