Epidemiology, Phenotypic and Genotypic Characterization of Carbapenem-Resistant Gram-Negative Bacteria from a Libyan Hospital.
Khouloud SlimeneAllaaeddin El SalabiOlfa DziriNajla MathlouthiSeydina M DieneElhussan Ahmed MohamedJadalla M A AmhalhalMohammed O AboalgasemJomaa F AlrjaelJean-Marc RolainChedly ChouchaniPublished in: Microbial drug resistance (Larchmont, N.Y.) (2023)
Antimicrobial resistance, particularly resistance to carbapenems, has become one of the major threats to public health. Seventy-two isolates were collected from patients and hospital environment of Ibn Sina Hospital, Sirte, Libya. Antibiotic susceptibility tests, using the disc diffusion method and E-Test strips, were performed to select carbapenem-resistant strains. The colistin (CT) resistance was also tested by determining the minimum inhibitory concentration (MIC). RT-PCR was conducted to identify the presence of carbapenemase encoding genes and plasmid-mediated mcr CT resistance genes. Standard PCR was performed for positive RT-PCR and the chromosome-mediated CT resistance genes ( mgrB, pmrA, pmrB, phoP, phoQ ). Gram-negative bacteria showed a low susceptibility to carbapenems. Molecular investigations indicated that the metallo-β-lactamase New Delhi metallo-beta-lactamases-1 was the most prevalent ( n = 13), followed by Verona integron-encoded metallo-beta-lactamase (VIM) enzyme (VIM-2 [ n = 6], VIM-1 [ n = 1], and VIM-4 [ n = 1]) that mainly detected among Pseudomonas spp . The oxacillinase enzyme OXA-23 was detected among six Acinetobacter baumannii , and OXA-48 was detected among one Citrobacter freundii and three Klebsiella pneumoniae, in which one coharbored the Klebsiella pneumoniae carbapenemase enzyme and showed resistance to CT (MIC = 64 μg/mL) by modification in pmrB genes. In this study, we report for the first time the emergence of Pseudomonas aeruginosa carrying the bla NDM-1 gene and belonging to sequence type773 in Libya. Our study reported also for the first time CT resistance by mutation in the pmrB gene among Enterobacteriaceae isolates in Libya.
Keyphrases
- klebsiella pneumoniae
- multidrug resistant
- escherichia coli
- acinetobacter baumannii
- gram negative
- genome wide
- pseudomonas aeruginosa
- drug resistant
- image quality
- computed tomography
- public health
- contrast enhanced
- genome wide identification
- dual energy
- antimicrobial resistance
- healthcare
- genome wide analysis
- magnetic resonance imaging
- positron emission tomography
- ejection fraction
- staphylococcus aureus
- biofilm formation
- magnetic resonance
- crispr cas
- gene expression
- dna methylation
- chronic kidney disease
- emergency department
- copy number
- acute care
- adverse drug
- candida albicans
- single molecule
- peritoneal dialysis
- plant growth
- amino acid