Electrochemical interface based on polydopamine and gold nanoparticles/reduced graphene oxide for impedimetric detection of lung cancer cells.
Nguyen Dieu LinhNguyen Thi Trang HuyenNguyen Hai DangBenoit PiroVu Thi ThuPublished in: RSC advances (2023)
The use of non-invasive approaches for monitoring therapy processes in cancer patients at late stages is truly needed. In this work, we aim to develop an electrochemical interface based on polydopamine combined with gold nanoparticles and reduced graphene oxide for impedimetric detection of lung cancer cells. Gold nanoparticles (around 75 nm) were dispersed onto reduced graphene oxide material pre-electrodeposited onto disposable fluorine doped tin oxide electrodes. The coordination between gold and carbonaceous material has somehow improved the mechanical stability of this electrochemical interface. Polydopamine was later introduced onto modified electrodes via self-polymerization of dopamine in an alkaline solution. The result has demonstrated the good adhesion and biocompatibility of polydopamine towards A-549 lung cancer cells. The presence of the two conductive materials (gold nanoparticles and reduced graphene oxide) has led to a six-times decrease in charge transfer resistance of polydopamine film. Finally, the as-prepared electrochemical interface was employed for impedimetric detection of A-549 cells. The detection limit was estimated to be only 2 cells per mL. These findings have proved the possibilities to use advanced electrochemical interfaces for point-of-care applications.
Keyphrases
- reduced graphene oxide
- gold nanoparticles
- label free
- loop mediated isothermal amplification
- induced apoptosis
- magnetic nanoparticles
- real time pcr
- cell cycle arrest
- computed tomography
- signaling pathway
- mesenchymal stem cells
- stem cells
- squamous cell carcinoma
- highly efficient
- cystic fibrosis
- positron emission tomography
- high resolution
- replacement therapy
- mass spectrometry
- cell migration
- photodynamic therapy
- endoplasmic reticulum stress