Viral envelope proteins fused to multiple distinct fluorescent reporters to probe receptor binding.
Ilhan TomrisRoosmarijn van der WoudeRebeca de Paiva Froes RochaAlba Torrents de la PeñaAndrew B WardRobert P de VriesPublished in: Protein science : a publication of the Protein Society (2024)
Enveloped viruses carry one or multiple proteins with receptor-binding functionalities. Functional receptors can be glycans, proteinaceous, or both; therefore, recombinant protein approaches are instrumental in attaining new insights regarding viral envelope protein receptor-binding properties. Visualizing and measuring receptor binding typically entails antibody detection or direct labeling, whereas direct fluorescent fusions are attractive tools in molecular biology. Here, we report a suite of distinct fluorescent fusions, both N- and C-terminal, for influenza A virus hemagglutinins and SARS-CoV-2 spike RBD. The proteins contained three or six fluorescent protein barrels and were applied directly to cells to assess receptor binding properties.