Preparation of near-infrared photoacoustic imaging and photothermal treatment agent for cancer using a modifiable acid-triggered molecular platform.
Xiaoming LiuChunbai XiangYalin LvJingjing XiangGongcheng MaChangzhong LiYan HuChunlei GuoHua SunLintao CaiPing GongPublished in: The Analyst (2024)
Ratiometric near-infrared fluorescent pH probes with various p K a values were innovatively designed and synthesized based on cyanine with a diamine moiety. The photochemical properties of these probes were thoroughly evaluated. Among the series, IR-PHA exhibited an optimal p K a value of approximately 6.40, closely matching the pH of cancerous tissues. This feature is particularly valuable for real-time pH monitoring in both living cells and living mice. Moreover, when administered intravenously to tumor-bearing mice, IR-PHA demonstrated rapid and significant enhancement of near-infrared fluorescence and photoacoustic signals within the tumor region. This outcome underscores the probe's exceptional capability for dual-modal cancer imaging utilizing near-infrared fluorescence (NIRF) and photoacoustic (PA) modalities. Concurrently, the application of a continuous-wave near-infrared laser efficiently ablated cancer cells in vivo , attributed to the photothermal effect induced by IR-PHA. The results strongly indicate that IR-PHA is well-suited for NIRF/PA dual-modality imaging and photothermal therapy of tumors. This makes it a promising candidate for theranostic applications involving small molecules.
Keyphrases
- living cells
- single molecule
- fluorescent probe
- fluorescence imaging
- photodynamic therapy
- high resolution
- papillary thyroid
- drug delivery
- cancer therapy
- squamous cell
- gene expression
- small molecule
- high fat diet induced
- type diabetes
- young adults
- squamous cell carcinoma
- adipose tissue
- insulin resistance
- sensitive detection
- molecularly imprinted
- deep learning
- label free
- skeletal muscle
- drug release
- loop mediated isothermal amplification