Integrating ion mobility into comprehensive multidimensional metabolomics workflows: critical considerations.
Jody C MayJohn A McLeanPublished in: Metabolomics : Official journal of the Metabolomic Society (2022)
We outline a strategy for unifying IM-derived collision cross section (CCS) measurements obtained from different IM techniques and discuss the emerging field of high resolution ion mobility (HRIM) that is poised to address many of the contemporary challenges associated with ion mobility metabolomics. Whereas the LC step limits the throughput of comprehensive LC-IM-MS, the higher peak capacity of HRIM can allow fast LC gradients or rapid sample cleanup via solid-phase extraction (SPE) to be utilized, significantly improving the sample throughput.
Keyphrases
- solid phase extraction
- mass spectrometry
- liquid chromatography
- simultaneous determination
- tandem mass spectrometry
- high performance liquid chromatography
- liquid chromatography tandem mass spectrometry
- high resolution
- molecularly imprinted
- gas chromatography mass spectrometry
- ultra high performance liquid chromatography
- high resolution mass spectrometry
- gas chromatography
- ms ms
- multiple sclerosis
- high speed
- loop mediated isothermal amplification