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Proximity ligation-transcription circuit-powered exponential amplifications for single-molecule monitoring of telomerase in human cells.

Li-Juan WangMeng-Meng LvJin-Ping HuMeng LiuShuangshuang Zhang
Published in: Chemical communications (Cambridge, England) (2023)
We develop a new strategy for single-molecule monitoring of telomerase based on proximity ligation-transcription circuit-powered exponential amplifications. This strategy exhibits high sensitivity with a detection limit of 0.1 aM for the synthetic telomerase product TPC4 in vitro and 1 HeLa cell in vivo . Moreover, it can screen potential inhibitors, discriminate telomerase from interferents, and distinguish cancer cells from normal cells.
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