Nitric oxide (NO) is reported to be elevated in osteoarthritis (OA) both in vitro and in vivo and may be adopted to develop fluorescent probes for detecting the progression of OA. Here we report a nitric oxide responsive aggregation induced emission (AIE) probe TPE-2NHCOCH 2 CH 2 -(PEG) 24 -NH-Diacerein, which is derived from tetraphenylethene (TPE) modified with the hydrophilic group long poly(ethylene glycol) chain and an anti-inflammatory drug diacerein. o -Phenylenediamine within its structure can react with NO to form benzotriazole and emit fluorescence. The results show that the NO-responsive AIE probe can smartly monitor the progression of OA with the change of fluorescence intensity in vitro and in vivo . This study may provide a new development direction for early OA monitoring in clinics.
Keyphrases
- living cells
- nitric oxide
- single molecule
- knee osteoarthritis
- fluorescent probe
- nitric oxide synthase
- cancer therapy
- hydrogen peroxide
- anti inflammatory
- rheumatoid arthritis
- quantum dots
- drug delivery
- primary care
- room temperature
- high intensity
- emergency department
- photodynamic therapy
- simultaneous determination
- drug induced
- label free