Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System.
Yujin AhnJu-Hyun AnHae-Jun YangDong Gil LeeJieun KimHyebin KohYoung-Ho ParkBong-Seok SongBo-Woong SimHong J LeeJong-Hee LeeSun-Uk KimPublished in: Cells (2021)
Vascularization of tissues, organoids and organ-on-chip models has been attempted using endothelial cells. However, the cultured endothelial cells lack the capacity to interact with other somatic cell types, which is distinct from developing vascular cells in vivo. Recently, it was demonstrated that blood vessel organoids (BVOs) recreate the structure and functions of developing human blood vessels. However, the tissue-specific adaptability of BVOs had not been assessed in somatic tissues. Herein, we investigated whether BVOs infiltrate human cerebral organoids and form a blood-brain barrier. As a result, vascular cells arising from BVOs penetrated the cerebral organoids and developed a vessel-like architecture composed of CD31+ endothelial tubes coated with SMA+ or PDGFR+ mural cells. Molecular markers of the blood-brain barrier were detected in the vascularized cerebral organoids. We revealed that BVOs can form neural-specific blood-vessel networks that can be maintained for over 50 days.
Keyphrases
- endothelial cells
- induced pluripotent stem cells
- induced apoptosis
- blood brain barrier
- high glucose
- subarachnoid hemorrhage
- cell cycle arrest
- vascular endothelial growth factor
- gene expression
- cerebral ischemia
- stem cells
- signaling pathway
- oxidative stress
- mesenchymal stem cells
- cell death
- copy number
- dna methylation
- single molecule
- atomic force microscopy