NMR-Based Metabolomics Revealed the Underlying Inflammatory Pathology in Reactive Arthritis Synovial Joints.
Durgesh DubeySandeep KumarAtul RawatAnupam GuleriaReena KumariSakir AhmedRajeev SinghShubhini A SarafDinesh KumarPublished in: Journal of proteome research (2021)
Reactive arthritis (ReA) is an aseptic synovitis condition that often develops 2-4 weeks after a distant (extra-articular) infection with Chlamydia, Salmonella, Shigella, Campylobacter, and Yersinia species. The metabolic changes in the synovial fluid (SF) may serve as indicative markers to both improve the diagnostic accuracy and understand the underlying inflammatory pathology of ReA. With this aim, the metabolic profiles of SF collected from ReA (n = 58) and non-ReA, i.e., rheumatoid arthritis (RA, n = 21) and osteoarthritis (OA, n = 20) patients, respectively, were measured using NMR spectroscopy and compared using orthogonal partial least-squares discriminant analysis (OPLS-DA). The discriminatory metabolic features were further evaluated for their diagnostic potential using the receiver operating characteristic (ROC) curve analysis. Compared to RA, two (alanine and carnitine), and compared to OA, six (NAG, glutamate, glycerol, isoleucine, alanine, and glucose) metabolic features were identified as diagnostic biomarkers. We further demonstrated the impact of ReA synovitis condition on the serum metabolic profiles through performing a correlation analysis. The Pearson rank coefficient (r) was estimated for 38 metabolites (profiled in both SF and serum samples obtained in pair from ReA patients) and was found significantly positive for 71% of the metabolites (r ranging from 0.17 to 0.87).
Keyphrases
- rheumatoid arthritis
- end stage renal disease
- newly diagnosed
- ejection fraction
- chronic kidney disease
- disease activity
- oxidative stress
- ms ms
- knee osteoarthritis
- prognostic factors
- peritoneal dialysis
- magnetic resonance
- mass spectrometry
- escherichia coli
- ankylosing spondylitis
- computed tomography
- single cell
- interstitial lung disease
- staphylococcus aureus
- cystic fibrosis
- lymph node
- systemic sclerosis
- climate change
- adipose tissue
- skeletal muscle
- preterm birth
- blood glucose