Multiple sclerosis (MS) is the most common autoimmune demyelinating disease of the central nervous system (CNS). Here, we have explored a novel use of glyceryl tribenzoate (GTB), a flavoring ingredient, in ameliorating the disease process of experimental allergic encephalomyelitis (EAE), an animal model of MS, via TGF-β. Oral feeding of GTB suppressed clinical symptoms of adoptively-transferred relapsing-remitting (RR) EAE in recipient mice and suppressed the generation of encephalitogenic T cells in donor mice. GTB also attenuated clinical symptoms of RR-EAE in PLP-TCR transgenic mice and chronic EAE in male C57/BL6 mice. Accordingly, GTB also suppressed perivascular cuffing, preserved the integrity of blood-brain barrier and blood-spinal cord barrier, inhibited inflammation, and stopped demyelination in the CNS of EAE mice. Interestingly, GTB treatment upregulated TGF-β and enriched regulatory T cells (Tregs) in splenocytes as well as in vivo in EAE mice. Blocking TGF-β by neutralizing antibodies abrogated GTB-mediated enrichment of Tregs and protection of EAE. These results suggest that oral GTB may be considered as a possible therapy for MS patients.
Keyphrases
- multiple sclerosis
- blood brain barrier
- regulatory t cells
- high fat diet induced
- transforming growth factor
- white matter
- end stage renal disease
- type diabetes
- spinal cord injury
- chronic kidney disease
- rheumatoid arthritis
- metabolic syndrome
- stem cells
- adipose tissue
- oxidative stress
- peritoneal dialysis
- mesenchymal stem cells
- skeletal muscle
- sleep quality
- disease activity
- allergic rhinitis
- physical activity
- replacement therapy
- patient reported
- atopic dermatitis