Processing of Holographic Hydrogels in Liquid Media: A Study by High-Performance Liquid Chromatography and Diffraction Efficiency.
Kheloud BerramdaneManuel G RamírezPaola ZezzaMaría Isabel LucíoMaría-José BañulsÁngel MaquieiraMarta Morales-VidalInmaculada PascualInmaculada PascualPublished in: Polymers (2022)
The storage of time-stable holographic gratings in hydrogel matrices when the material is immersed in aqueous media is a real challenge at present. The optimization of the storage stages of the holograms must be properly investigated to identify the most suitable development processes. For this reason, this work is focused on the study of the optimization of the washing stages of the hydrogels based on acrylamide and N , N '-methylenebis(acrylamide) once unslanted transmission holograms have been stored. High-performance liquid chromatography and UV-visible measurements have been employed in our system to analyze the composition of the washing solutions. PBST and DMSO:H 2 O are used as solvents in the washing stages. The diffraction efficiencies are measured during the washing stages and after the storing of the holograms during several days in PBST. Maximum diffraction efficiencies of 38 and 27.6% are reached when PBST and DMSO:H 2 O are employed, respectively, for the washing process. Holograms show temporal stability after being stored immersed in PBST at 4 °C for 4 days.
Keyphrases
- high performance liquid chromatography
- tandem mass spectrometry
- mass spectrometry
- solid phase extraction
- simultaneous determination
- drug delivery
- ionic liquid
- hyaluronic acid
- room temperature
- crystal structure
- liquid chromatography
- tissue engineering
- extracellular matrix
- drug release
- high resolution
- ms ms
- atomic force microscopy