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Immunoassay approach for diagnosis of exposure to pyrrolizidine alkaloids.

Na LiFan ZhangWei LianHuali WangJiang ZhengGe Lin
Published in: Journal of environmental science and health. Part C, Environmental carcinogenesis & ecotoxicology reviews (2017)
Numerous pyrrolizidine alkaloid (PA) poisoning cases have been documented worldwide. Protein covalent binding with reactive metabolites generated from metabolic activation of PAs to form pyrrole-protein adducts is suggested to be a primary mechanism of PA-induced toxicities. The present study aimed to develop antibodies for diagnosis of PA exposure. Polyclonal antibodies were raised in rabbits and proven to specifically recognize pyrrole-protein adducts regardless of amino acid residues modified by the reactive metabolites of PAs. The developed antibodies were successfully applied to detect pyrrole-protein adducts in blood samples obtained from PA-treated rats and exhibited a potential for the clinical diagnosis of PA exposure.
Keyphrases
  • amino acid
  • protein protein
  • binding protein
  • ms ms
  • risk assessment
  • sensitive detection
  • climate change
  • dna binding
  • endothelial cells