RNA Sequencing Experimental Analysis Workflow Using Caenorhabditis elegans.

Jose RobledoSaifun Ripa NaharManuel A RuizRaymond J HendricksDavid J BurksMary L LadageTaegun KwonRajeev K AzadPamela A Padilla

Published in: Methods in molecular biology (Clifton, N.J.) (2024)

RNA sequencing is an approach to transcriptomic profiling that enables the detection of differentially expressed genes in response to genetic mutation or experimental treatment, among other uses. Here we describe a method for the use of a customizable, user-friendly bioinformatic pipeline to identify differentially expressed genes in RNA sequencing data obtained from C. elegans, with attention to the improvement in reproducibility and accuracy of results.

Keyphrases

single cell
rna seq
genome wide
electronic health record
bioinformatics analysis
genome wide identification
working memory
dna methylation
copy number
big data
combination therapy
real time pcr
machine learning
label free
transcription factor