Overcome Isomer Interference in 1α,25-Dihydroxyvitamin D Quantitation by Liquid Chromatography-Tandem Mass Spectrometry.

Zhicheng JinRoger L BertholfXin Yi

Published in: The journal of applied laboratory medicine (2021)

The LC-MS/MS method we developed without an immunoextraction procedure was able to resolve the major interference peak from 1α,25(OH)2D and achieved reliable quantitation of 1α,25(OH)2D.

Keyphrases

liquid chromatography tandem mass spectrometry
simultaneous determination
ms ms
solid phase extraction
minimally invasive
high performance liquid chromatography
tandem mass spectrometry
mass spectrometry
high resolution