Login / Signup

Bioluminescent-based imaging and quantification of glucose uptake in vivo.

Tamara MaricGeorgy MikhaylovPavlo KhodakivskyiArkadiy A BazhinRiccardo SinisiNicolas BonhoureAleksey YevtodiyenkoAnthony JonesVishaka MuhunthanGihad AbdelhadyDavid ShackelfordElena A Goun
Published in: Nature methods (2019)
Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used 18F-FDG-positron-emission-tomography tracers and validate the bioluminescent glucose-uptake probe as a tool for the identification of new glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the fields of metabolism and drug development.
Keyphrases
  • positron emission tomography
  • high resolution
  • blood glucose
  • computed tomography
  • pet imaging
  • pet ct
  • type diabetes
  • quantum dots
  • mass spectrometry
  • fluorescence imaging
  • living cells
  • high speed
  • single molecule