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AcrB-AcrA Fusion Proteins That Act as Multidrug Efflux Transporters.

Katsuhiko HayashiRyosuke NakashimaKeisuke SakuraiKimie KitagawaSeiji YamasakiKunihiko NishinoAkihito Yamaguchi
Published in: Journal of bacteriology (2015)
The structure and stoichiometry of the RND-type multidrug exporter AcrB-AcrA-TolC complex are still under debate. Recently, electron microscopic images of the AcrB-AcrA-TolC complex have been reported, suggesting a 1:2:1 stoichiometry. However, we report here that the AcrB-AcrA 1:1 fusion protein is active for drug export under acrAB-deficient conditions and also under acrAB acrEF double-deficient conditions, which eliminate the aid of free AcrA and its close homolog AcrE, indicating that the AcrB-AcrA 1:1 stoichiometry is enough for drug export function. In addition, the AcrB-AcrA fusion protein can function without the aid of free AcrA. We believe that these results are very important for considering the structure and mechanism of AcrAB-TolC-mediated multidrug export.
Keyphrases
  • drug resistant
  • emergency department
  • optical coherence tomography
  • drug induced
  • adverse drug