Total Dissolved Inorganic Carbon Sensor Based on Amperometric CO2 Microsensor and Local Acidification.

We present a dipping probe total dissolved inorganic carbon (DIC) microsensor based on a localized acidic microenvironment in front of an amperometric CO2 microsensor. The acidic milieu facilitates conversion of bicarbonate and carbonate to CO2, which in turn is reduced at a silver cathode. Interfering oxygen is removed by an acidic CrCl2 oxygen trap. Theoretical simulations of microsensor functioning were performed to find a suitable compromise between response time and near-complete conversion of bicarbonate to CO2. The sensor exhibited a linear response over a wide range of 0-8 mM DIC, with a calculated LOD of 5 μM and a 90% response time of 150 s. The sensor was successfully tested in measuring DIC in bottled mineral water and seawater. This DIC microsensor holds the potential to become an important tool in environmental sensing and beyond for measurements of DIC at high spatial and temporal resolution.