Increased levels of pathogenic Th17 cells and diminished function of CD69+ Treg lymphocytes in patients with overweight.
Alejandra Mendoza-PérezMarlen Vitales-NoyolaLarisa González-BarandaCrisol Álvarez-QuirogaBerenice Hernández-CastroAdriana Monsiváis-UrendaLourdes BarandaPerla Niño-MorenoGilberto HurtadoRaquel Sánchez-GutiérrezRoberto González-AmaroPublished in: Clinical and experimental immunology (2022)
A low-grade inflammatory phenomenon is a feature of overweight and metabolic syndrome. The involvement of a pro-inflammatory Th17 lymphocyte subset and the CD69+ T regulatory (Treg) cell subtype in patients with metabolic dysfunction associated with or without overweight has not been fully elucidated. The aim of this study was to perform a quantitative and functional analysis of pathogenic Th17 lymphocytes and CD69+ Treg cells in patients with metabolic dysfunction (insulin resistance and dyslipidemia). The number of pathogenic Th17 cells and the levels and function of CD69+ Treg cells were analyzed in blood samples from individuals with metabolic dysfunction, associated with or without overweight. Pathogenic and non-pathogenic Th17 lymphocytes as well as Th22 cells were determined by eight-color flow cytometry analysis, whereas the levels and suppressive function of CD69+ Treg cells were also analyzed by multiparametric flow cytometry. We detected increased levels of pro-inflammatory Th17 pathogenic cells and Th22 lymphocytes in overweight unhealthy individuals (P < 0.001, compared to normal weight healthy). Conversely, diminished numbers of CD69+ Treg lymphocytes were observed in metabolically unhealthy individuals, with or without overweight. Likewise, the immunosuppressive function of CD69+ Treg cells was also defective in these patients. The increased levels of pathogenic Th17 cells along with a diminished number and function of CD69+ Treg lymphocytes may significantly contribute to the low-grade inflammatory phenomenon of metabolically unhealthy patients.
Keyphrases
- induced apoptosis
- cell cycle arrest
- low grade
- metabolic syndrome
- oxidative stress
- insulin resistance
- weight loss
- flow cytometry
- physical activity
- end stage renal disease
- type diabetes
- signaling pathway
- peripheral blood
- body mass index
- transcription factor
- chronic kidney disease
- mesenchymal stem cells
- stem cells
- machine learning
- adipose tissue
- prognostic factors
- cell therapy
- pi k akt
- skeletal muscle
- polycystic ovary syndrome