A gel-based PCR method to differentiate sheeppox virus field isolates from vaccine strains.
Tesfaye Rufael ChibssaReingard GrabherrAngelika LoitschTirumala Bharani K SettypalliEeva TuppurainenNick NwankpaKarim TounkaraHafsa MadaniAmel OmaniMariane DiopGiovanni CattoliAdama DialloCharles Euloge LamienPublished in: Virology journal (2018)
The developed assay is an improvement of currently existing diagnostic tools and, when combined with a capripox virus species-specific assay, will enhance SPP and GTP diagnosis and surveillance and facilitate epidemiological investigations in countries using live attenuated SPP vaccines. In addition, for laboratories with limited resources, the assay provides a simple and cost-effective alternative for sequencing.